An Amperometric Immunosensor Based on Multi-Walled Carbon Nanotubes-Thionine-Chitosan Nanocomposite Film for Chlorpyrifos Detection
نویسندگان
چکیده
In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surface of MWCNTs-THI-CHIT/GCE using the crosslinking agent glutaraldehyde (GA). The modification procedure was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, a linear relationship between the relative change in peak current of different pulse voltammetry (DPV) and the logarithm of chlorpyrifos solution concentration was obtained in the range from 0.1 to 1.0 × 10(5) ng/mL with a detection limit of 0.046 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, making it a potential alternative tool for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.
منابع مشابه
Electroanalytical sensing of Asulam based on nanocomposite modified glassy carbon electrode
In this study a facile approach to employ Copper nanoparticle (CuNPs) and multi-walled carbon nanotubes (MWCNT) as the nanomaterial for selective detection of asulam have been investigated. This work reports the electrocatalytic oxidation of asulam on glassy carbon electrodes (GCE) modified with multi-walled carbon nanotubes (MWCNT), ionic liquids (IL), chitosan (Chit) and copper nanoparticles ...
متن کاملA Label-Free Microelectrode Array Based on One-Step Synthesis of Chitosan–Multi-Walled Carbon Nanotube–Thionine for Ultrasensitive Detection of Carcinoembryonic Antigen
Carcinoembryonic antigen (CEA) has been an extensively used tumor marker responsible for clinical early diagnosis of cervical carcinomas, and pancreatic, colorectal, gastric and lung cancer. Combined with micro-electro mechanical system (MEMS) technology, it is important to develop a novel immune microelectrode array (MEA) not only for rapid analysis of serum samples, but also for cell detectio...
متن کاملA Portable Pesticide Residues Detection Instrument Based on Impedance Immunosensor
In this paper, a design of portable pesticide residues detection instrument was presented based on an impedance immunosensor. The immunosensor exploited the novel multilayer films based on Au nanoparticles (AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite (PANI/MWCNTs/CS). The detection principle of the instrument was based on the electrochemical characteris...
متن کاملBiosensor with L-phenylalanine Dehydrogenase and Diaphorase Immobilized on Nanocomposite Electrode for the Determination of L-phenylalanine in Urine
Bienzymatic amperometric biosensor consisting of L-phenylalanine dehydrogenase and diaphorase for the determination of L-phenylalanine has been developed. Enzymes were immobilized between chitosan layers onto the surface of planar nanocomposite electrodes based on multi-walled carbon nanotubes. Wide linear concentration range was obtained for 12–1250 μM of L-phenylalanine in phosphate buffer so...
متن کاملFunctionalized single-walled carbon nanotubes/polypyrrole composites for amperometric glucose biosensors
This article reports an amperometric glucose biosensor based on a new type of nanocomposite of polypyrrole (PPY) with p-phenyl sulfonate-functionalized single-walled carbon nanotubes (SWCNTs-PhSO3-). An environmentally friendly functionalization procedure of the SWCNTs in the presence of substituted aniline and an oxidative species was adopted. The nanocomposite-modified electrode exhibited exc...
متن کامل